Stem Pharm’s synthetic substrate technology is based on functionalized multi-armed polyethylene glycol monomers that are cross-linked and incorporate bioactive peptides to create optimized adhesion and biomechanical properties for cell culture applications. These optically clear substrates lack the confounding complexities of animal-derived substrates, and generate appropriate cellular environments that improve outcomes for cell expansion, differentiation, and in vitro cell-based assays.
SureCoat Pre-Coated Cell Culture Plates
TM
Culture plates pre-coated with Stem Pharm’s SureCoat synthetic substrate
Optimized for superior cell adhesion
Ready-to-use out of the package
Shelf stable at room temperature
Accelerates cell maturation, enhances cellular phenotypes and improves assay performance
Designed for iPSC-derived neural cells, and other cell types including iPSCs, MSCs and endothelial cells
Induced excitatory neurons grown on SureCoat synthetic substrate exhibiting excellent adhesion and morphology in long-term cultures.
iPSC-derived motor neurons cultured on SureCoat synthetic substrate exhibiting increased synaptophysin expression and enhanced neurite outgrowth compared to culturing on PDL.
Ready-To-Dispense Hydrogels
Contact us at info@stempharm.com for purchasing information
Chemically defined hydrogel
Non-toxic to cells
Compatible with automation workflows; not temperature sensitive
Supplied frozen
Optically clear
Applications include:
Reproducible generation of organoids
Encapsulation of tumor organoids and spheroids
Development of high quality in vitro cell-based assays
Two ready-to-dispense formulations available:
SP-105
Designed for in vitro cellular assays
Formulation optimized for cell adhesion
Application – high quality vascular tubulogenesis assays with HUVEC and iPSC-derived endothelial cells
SP-111
Designed for encapsulation and expansion of spheroids and organoids
Supplied concentrated at 2X and can be diluted to create an optimized supportive biomechanical environment
We also develop custom synthetic hydrogels optimized for your cell application
Application: SP-105 Enabled Vascular Tubulogenesis Assay
Human Vascular Endothelial Cells (HUVEC) plated onto Stem Pharm hydrogel and stimulated with VEGF to form vascular tubulogenic structures.
Vascular tubulogenic structures can be quantified through image analysis. Dose response curves of vascular disruptors Nocodazole and Axitinib were tested with HUVEC cells and quantified at 24 h.
Synthetic alternatives to Matrigel
Aisenbrey & Murphy (2021)
Versatile synthetic alternatives to Matrigel for vascular toxicity screening and stem cell expansion
Nguyen, et al. (2017)